Application

The unconjugated antibody (Cat. No. MABN92) is shown to be suitable also for Western blotting and immunohistochemistry applications.

Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor^® 647 Conjugate is an antibody against AIF1 for use in Immunofluorescence.

General description

Allograft inflammatory factor 1 (UniProt P55008; also known as AIF-1, Daintain, Interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, Protein G1) is encoded by the AIF1 (also known as G1, IBA1) gene (Gene ID 199) in human. Iba1/AIF1 is a 17 kDa interferon-gamma- (IFN-gamma-) inducible, Ca(2+)-binding EF-hand protein. Iba1/AIF1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and macrophage activation/function. It is also shown to be expressed in microglial cells in autominnume disesase models such as experimental autoimmune encephalomyelitis, neuritis and uveitis.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Quality

Evaluated by Immunofluorescence in human spleen tissue.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected Iba1/AIF1 in human spleen tissue.

Target description

~17 kDa observed.